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Research Papers: Fuel Combustion

Genotoxicity of Diesel Particulate Matter Emitted by Port-Injection of Hydrous Ethanol and n-Butanol

[+] Author and Article Information
Marlon Cadrazco

Department of Mechanical Engineering,
Universidad de Antioquia (UdeA),
Calle 70 No. 52-21,
Medellín 050010, Colombia
e-mail: Marlon.cadrazco@udea.edu.co

John R. Agudelo

Department of Mechanical Engineering,
Universidad de Antioquia (UdeA),
Calle 70 No. 52-21,
Medellín 050010, Colombia
e-mail: John.agudelo1@udea.edu.co

Luz Y. Orozco

Environmental Modeling and
Management (GAIA),
Universidad de Antioquia (UdeA),
Calle 70 No. 52-21,
Medellín 050010, Colombia
e-mail: lyorozcoj@gmail.com

Verónica Estrada

Environmental Modeling and
Management (GAIA),
Universidad de Antioquia (UdeA),
Calle 70 No. 52-21,
Medellín 050010, Colombia
e-mail: veronicae77@gmail.com

Contributed by the Internal Combustion Engine Division of ASME for publication in the JOURNAL OF ENERGY RESOURCES TECHNOLOGY. Manuscript received February 20, 2017; final manuscript received February 28, 2017; published online March 30, 2017. Assoc. Editor: Stephen A. Ciatti.

J. Energy Resour. Technol 139(4), 042207 (Mar 30, 2017) (5 pages) Paper No: JERT-17-1088; doi: 10.1115/1.4036253 History: Received February 20, 2017; Revised February 28, 2017

This work evaluated the genotoxic potential of the soluble organic material (SOM) extracted from the particulate matter (PM) emitted by an automotive diesel engine. The engine was modified to operate with a home-made multipoint-port injection system to substitute 10% of ultralow-sulfur diesel (ULSD) fuel in energy basis by hydrous ethanol (h-Et) or n-butanol (n-Bu) injected into the manifold during the intake stroke. A low engine load mode named M4 (43 N·m at 2410 min−1) and a medium-load mode M2 (95 N·m at 2410 min−1) were selected from the vehicle homologation cycle. PM was collected with a stainless steel filter located 1.5 m downstream the exhaust manifold. The SOM of the PM was extracted to evaluate the genotoxic activity on human lymphocytes using the comet assay. Results indicated that independently of the mode, the SOM coming from alcohols led more genotoxicity than ULSD, following the order h-Et > n-Bu > ULSD. The low engine load operation exhibited much more deoxyribonucleic acid (DNA) damage than mode M2, especially the PM produced by hydrous ethanol port-injection. Although further research is still necessary, these findings suggest that the biology activity of the SOM coming from alcohols PM could be a barrier for the implementation of alcohol port-injection technology.

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References

Maricq, M. M. , 2007, “ Chemical Characterization of Particulate Emissions From Diesel Engines: A Review,” J. Aerosol Sci., 38(11), pp. 1079–1118. [CrossRef]
EPA, 2002, “ Health Assessment Document for Diesel Engine Exhaust,” U.S. Environmental Protection Agency, Washington, DC, accessed Mar. 10, 2016, hero.epa.gov/index.cfm/reference/download/reference_id/42866
Bünger, J. , Krahl, J. , Schröder, O. , Schmidt, L. , and Westphal, G. A. , 2012, “ Potential Hazards Associated With Combustion of Bio-Derived Versus Petroleum-Derived Diesel Fuel,” Crit. Rev. Toxicol., 42(9), pp. 732–750. [CrossRef] [PubMed]
International Agency for Research on Cancer, 2012, “  IARC: Diesel Engine Exhaust Carcinogenic,” World Health Organization, Lyons, France.
Agarwal, A. K. , 2007, “ Biofuels (Alcohols and Biodiesel) Applications as Fuels for Internal Combustion Engines,” Prog. Energy Combust. Sci., 33(3), pp. 233–271. [CrossRef]
Lapuerta, M. , Armas, O. , and Rodríguez-Fernández, J. , 2008, “ Effect of Biodiesel Fuels on Diesel Engine Emissions,” Prog. Energy Combust. Sci., 34(2), pp. 198–223. [CrossRef]
Williams, A. , Black, S. , and McCormick, R. L. , 2010, “ Biodiesel Fuel Property Effects on Particulate Matter Reactivity,” 6th International Exhaust Gas and Particulate Emissions Forum, Ludwigsburg, Germany, Mar. 9–10, Paper No. NREL/CP-540-47286.
Giakoumis, E. G. , Rakopoulos, C. D. , Dimaratos, A. M. , and Rakopoulos, D. C. , 2013, “ Exhaust Emissions With Ethanol or N-Butanol Diesel Fuel Blends During Transient Operation: A Review,” Renewable Sustainable Energy Rev., 17, pp. 170–190. [CrossRef]
Imran, A. , Varman, M. , Masjuki, H. H. , and Kalam, M. A. , 2013, “ Review on Alcohol Fumigation on Diesel Engine: A Viable Alternative Dual Fuel Technology for Satisfactory Engine Performance and Reduction of Environment Concerning Emission,” Renewable Sustainable Energy Rev., 26, pp. 739–751. [CrossRef]
Surawski, N. C. , Ristovski, Z. D. , Brown, R. J. , and Situ, R. , 2012, “ Gaseous and Particle Emissions From an Ethanol Fumigated Compression Ignition Engine,” Energy Convers. Manage., 54(1), pp. 145–151. [CrossRef]
Zhang, Z. H. , Cheung, C. S. , and Yao, C. D. , 2013, “ Influence of Fumigation Methanol on the Combustion and Particulate Emissions of a Diesel Engine,” Fuel, 111, pp. 442–448. [CrossRef]
Chen, Z. , Liu, J. , Wu, Z. , and Lee, C. , 2013, “ Effects of Port Fuel Injection (PFI) of N-Butanol and EGR on Combustion and Emissions of a Direct Injection Diesel Engine,” Energy Convers. Manage., 76, pp. 725–731. [CrossRef]
López, A. F. , Cadrazco, M. , Agudelo, A. F. , Corredor, L. A. , Vélez, J. A. , and Agudelo, J. R. , 2015, “ Impact of N-Butanol and Hydrous Ethanol Fumigation on the Performance and Pollutant Emissions of an Automotive Diesel Engine,” Fuel, 153, pp. 483–491. [CrossRef]
Broukhiyan, E. , and Lestz, S. , 1981, “ Ethanol Fumigation of a Light Duty Automotive Diesel Engine,” SAE Technical Paper No. 811209.
Heisey, J. , and Lestz, S. , 1981, “ Aqueous Alcohol Fumigation of a Single-Cylinder Di Diesel Engine,” SAE Technical Paper No. 811208.
Houser, K. , Lestz, S. , Dukovich, M. , and Yasbin, R. , 1980, “ Methanol Fumigation of a Light Duty Automotive Diesel Engine,” SAE Technical Paper No. 801379.
Surawski, N. C. , Miljevic, B. , Roberts, B. A. , Modini, R. L. , Situ, R. , Brown, R. J. , Bottle, S. E. , and Ristovski, Z. D. , 2009, “ Particle Emissions, Volatility, and Toxicity From an Ethanol Fumigated Compression Ignition Engine,” Environ. Sci. Technol., 44(1), pp. 229–235. [CrossRef]
Stein, F. , 1960, “ The Integral Isobaric Heat of Vaporization of Mixtures,” Ph.D. thesis, The University of Michigan, Ann Arbor, MI.
Ruiz, F. A. , Cadrazco, M. , López, A. F. , Sanchez-Valdepeñas, J. , and Agudelo, J. R. , 2015, “ Impact of Dual-Fuel Combustion With N-Butanol or Hydrous Ethanol on the Oxidation Reactivity and Nanostructure of Diesel Particulate Matter,” Fuel, 161, pp. 18–25. [CrossRef]
Sato, M. , Umbuzeiro, G. A. , Coimbrao, C. , Coelho, M. , Sanchez, P. , Alonso, C. , and Martins, M. , 1995, “ Mutagenicity of Airborne Particulate Organic Material From Urban and Industrial Areas of Sao Paulo, Brazil,” Mutat. Res., 335(3), pp. 317–330. [CrossRef] [PubMed]
Umbuzeiro, G. A. , Franco, A. , Martins, M. H. , Kummrow, F. , Carvalho, L. , Schmeiser, H. H. , Leykauf, J. , Stiborova, M. , and Claxton, L. D. , 2008, “ Mutagenicity and DNA Adduct Formation of Pah, Nitro-Pah, and Oxy-Pah Fractions of Atmospheric Particulate Matter From São Paulo, Brazil,” Mutat. Res./Genet. Toxicol. Environ. Mutagen., 652(1), pp. 72–80. [CrossRef]
Noble, P. B. , and Cutts, J. H. , 1967, “ Separation of Blood Leukocytes by Ficoll Gradient,” Can. Vet. J., 8(5), pp. 110–111. [PubMed]
Mendoza, L. C. , Orozco, L. Y. , Zapata, L. M. , and Palacio, J. A. , 2013, “ Genotoxicidad Sobre Linfocitos Humanos Expuestos a Pm10 De Tres Sitios Del Valle De Aburrá (Antioquia),” Rev. Salud Pública, 15(2), pp. 294–306.
Platel, A. , Gervais, V. , Sajot, N. , Nesslany, F. , Marzin, D. , and Claude, N. , 2010, “ Study of Gene Expression Profiles in Tk6 Human Cells Exposed to DNA-Oxidizing Agents,” Mutat. Res./Fundam. Mol. Mech. Mutagen., 689(1–2), pp. 21–49. [CrossRef]
Hartmann, A. , Schumacher, M. , Plappert-Helbig, U. , Lowe, P. , Suter, W. , and Mueller, L. , 2004, “ Use of the Alkaline In Vivo Comet Assay for Mechanistic Genotoxicity Investigations,” Mutagenesis, 19(1), pp. 51–59. [CrossRef] [PubMed]
Brendler-Schwaab, S. , Hartmann, A. , Pfuhler, S. , and Speit, G. , 2005, “ The In Vivo Comet Assay: Use and Status in Genotoxicity Testing,” Mutagenesis, 20(4), pp. 245–254. [CrossRef] [PubMed]
Zúñiga, L. A. , 2009, “ Optimizaciones Metodológicas Del Ensayo Del Cometa Y Su Aplicación En Biomonitorización Humana,” Ph.D. thesis, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
Singh, N. P. , McCoy, M. T. , Tice, R. R. , and Schneider, E. L. , 1988, “ A Simple Technique for Quantitation of Low Levels of DNA Damage in Individual Cells,” Exp. Cell Res., 175(1), pp. 184–191. [CrossRef] [PubMed]
McNamee, J. P. , McLean, J. R. N. , Ferrarotto, C. L. , and Bellier, P. V. , 2000, “ Comet Assay: Rapid Processing of Multiple Samples,” Mutat. Res./Genet. Toxicol. Environ. Mutagen., 466(1), pp. 63–69. [CrossRef]
Ferreiro, G. R. , Badías, L. C. , Lopez-Nigro, M. , Palermo, A. , Mudry, M. , Elio, P. G. , and Carballo, M. A. , 2002, “ DNA Single Strand Breaks in Peripheral Blood Lymphocytes Induced by Three Nitroimidazole Derivatives,” Toxicol. Lett., 132(2), pp. 109–115. [CrossRef] [PubMed]
Zhang, Z. H. , Cheung, C. S. , Chan, T. L. , and Yao, C. D. , 2010, “ Experimental Investigation of Regulated and Unregulated Emissions From a Diesel Engine Fueled With Euro V Diesel Fuel and Fumigation Methanol,” Atmos. Environ., 44(8), pp. 1054–1061. [CrossRef]
Van Gerpen, J. H. , and Van Meter, D. , 1990, Emission Control in Diesel Engines by Alcohol Fumigation, Midwest Transportation Center, Ames, IA.
Song, C.-L. , Zhou, Y.-C. , Huang, R.-J. , Wang, Y.-Q. , Huang, Q.-F. , Lü, G. , and Liu, K.-M. , 2007, “ Influence of Ethanol–Diesel Blended Fuels on Diesel Exhaust Emissions and Mutagenic and Genotoxic Activities of Particulate Extracts,” J. Hazard. Mater., 149(2), pp. 355–363. [CrossRef] [PubMed]
Eastwood, P. , 2008, Particulate Emissions From Vehicles, Vol. 161, John Wiley & Sons, New York, Chap. 5.

Figures

Grahic Jump Location
Fig. 1

Length of the comet tail when apply (a) PBS, (b) DMSO, (c) H2O2, and (d) SOM from M4 h-Et

Grahic Jump Location
Fig. 2

Percentage of the damaged cells in modes M2 (top) and M4 (bottom)

Grahic Jump Location
Fig. 3

Weighted damage index for modes M2 (top) and M4 (bottom)

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