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Keywords: C2C12
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Proceedings Papers

Proc. ASME. FEDSM2021, Volume 3: Fluid Mechanics; Micro and Nano Fluid Dynamics; Multiphase Flow, V003T07A006, August 10–12, 2021
Paper No: FEDSM2021-65639
... direction of the ridge and the direction of the tangential force, were set: 0, 1. INTRODUCTION 45, and 90 degrees in each region. Myoblasts (C2C12: mouse Biological cells gather to form tissue. Orientation of cells is myoblast cell line) were used in the experiment. The scaffold plate with cells was set...
Proceedings Papers

Proc. ASME. FEDSM2021, Volume 3: Fluid Mechanics; Micro and Nano Fluid Dynamics; Multiphase Flow, V003T07A002, August 10–12, 2021
Paper No: FEDSM2021-65207
.... Myoblasts (C2C12: mouse myoblast cell line) were used in the test. After cultivation without flow for 24 hours for adhesion of the cells to the lower disk, constant τ was applied to the cells in the incubator for 7 days. The behavior of each cell during shear was tracked by time-lapse images observed...
Proceedings Papers

Proc. ASME. FEDSM2021, Volume 3: Fluid Mechanics; Micro and Nano Fluid Dynamics; Multiphase Flow, V003T07A001, August 10–12, 2021
Paper No: FEDSM2021-65204
... of the electrodes, the amplitude and frequency of the alternating current. The suspension of C2C12 (mouse myoblast cell line) was injected into the channel, and the movement of each flowing cell was analyzed at the microscopic movie image. A pair of titanium-coated (200 nm thick) asymmetric surface electrodes...
Proceedings Papers

Proc. ASME. FEDSM2021, Volume 3: Fluid Mechanics; Micro and Nano Fluid Dynamics; Multiphase Flow, V003T05A007, August 10–12, 2021
Paper No: FEDSM2021-65205
... parallel walls (a lower stationary culture disk, and an upper rotating disk) with a constant gap. The wall shear stress was controlled by the rotating speed of the upper disk. Four types of cells were used in the test: C2C12 (mouse myoblast cell line), HUVEC (Human Umbilical Vein Endothelial Cells), 3T3-L1...
Proceedings Papers

Proc. ASME. FEDSM2021, Volume 3: Fluid Mechanics; Micro and Nano Fluid Dynamics; Multiphase Flow, V003T07A004, August 10–12, 2021
Paper No: FEDSM2021-65211
.... Myoblasts (C2C12: mouse myoblast line) were used in the flow test. The main flow velocity of the medium (0.02 mm/s < v x < 0.23 mm/s) was controlled by the pressure difference between the inlet and the outlet. The shape of each flowing cell was tracked in a movie recorded by a camera attached...
Topics: Flow (Dynamics)
Proceedings Papers

Proc. ASME. FEDSM2021, Volume 3: Fluid Mechanics; Micro and Nano Fluid Dynamics; Multiphase Flow, V003T07A003, August 10–12, 2021
Paper No: FEDSM2021-65209
.... Myoblasts (C2C12: mouse myoblast cell line) were used for the test. The flow rate of the medium, in which the cells were suspended, was controlled by a pressure head between the inlet and the outlet. Deformation of each cell passing through the micro-slit was observed with an inverted phase contrast...
Proceedings Papers

Proc. ASME. FEDSM2020, Volume 2: Fluid Mechanics; Multiphase Flows, V002T03A011, July 13–15, 2020
Paper No: FEDSM2020-20075
... stationary culture plate and the upper rotating parallel plate to make a Couette type of the shear flow. By the rotating speed of the upper disk, the wall shear stress on the lower culture plate was controlled. C2C12 (mouse myoblast cell) was used in the test. After cultivation without flow for 24 hours...
Proceedings Papers

Proc. ASME. FEDSM2020, Volume 3: Computational Fluid Dynamics; Micro and Nano Fluid Dynamics, V003T06A004, July 13–15, 2020
Paper No: FEDSM2020-20073
...-ridge on the transparent polydimethylsiloxane plate and the micro-groove on the glass plate. C2C12 (mouse myoblast cells) was used in the test. The flow of suspension of cells was controlled by the pressure head between the inlet and the outlet. Deformation of cells passing through the micro slit...
Proceedings Papers

Proc. ASME. FEDSM2020, Volume 3: Computational Fluid Dynamics; Micro and Nano Fluid Dynamics, V003T06A005, July 13–15, 2020
Paper No: FEDSM2020-20096
... been constructed between two transparent PDMS disks. C2C12 (mouse myoblast cell line) was used in the test. A flow velocity (0.1 mm/s < v x < 2.4 mm/s) of the suspension of cells was controlled by the pressure difference between the inlet and the outlet. The shifted distance of each cell along...