Does cell orientation depend on the cell type in the shear stress field? Does that tendency change after the division? In this study, the behavior of each cell after division was tracked by time-lapse microscopic images through 24 hours of culture under a shear stress field. A constant shear stress field was applied to the cells in the Couette flow between the parallel walls: the lower static culture disc and the upper rotating disc. For comparison, four types of cells were used: C2C12 (mouse myoblast), HUVEC (human umbilical vein endothelial cells), 3T3-L1 (mouse adipose progenitor cells), and L929 (mouse fibroblast). The result is as follows. In the wall shear stress field of 1 Pa, HUVEC is oriented parallel to the flow, regardless of the division. In other cell types (C2C12, 3T3-L1, and L929) after division, the deformed cell tends to tilt to the direction parallel to the flow. The experimental results are expected to be applied to engineered tissue technologies.