Research Papers: Energy Systems Analysis

Growth Media Affects Microalgae Susceptibility to Disruption by Low-Frequency Power Ultrasound

[+] Author and Article Information
Rory Klinger

Department of Civil, Construction,
and Environmental Engineering,
San Diego State University,
5500 Campanile Drive,
San Diego, CA 92182-1326
e-mail: roryklinger@gmail.com

Temesgen Garoma

Department of Civil, Construction,
and Environmental Engineering,
San Diego State University,
5500 Campanile Drive,
San Diego, CA 92182-1326
e-mail: tgaroma@mail.sdsu.edu

Contributed by the Advanced Energy Systems Division of ASME for publication in the JOURNAL OF ENERGY RESOURCES TECHNOLOGY. Manuscript received December 22, 2017; final manuscript received July 31, 2018; published online August 30, 2018. Assoc. Editor: David Macphee.

J. Energy Resour. Technol 140(12), 122004 (Aug 30, 2018) (5 pages) Paper No: JERT-17-1727; doi: 10.1115/1.4041090 History: Received December 22, 2017; Revised July 31, 2018

The effect of microalgae growth medium on power ultrasound treatment of microalgal biomass was investigated. Chlorella vulgaris was grown in Bold's basal medium (BBM), Bristol's medium, sueoka medium, and MiracleGro All Purpose Water Soluble Plant Food. These media showed statistically indistinguishable intrinsic growth rates, averaging 0.052/day. Power ultrasound treatment was applied at 9.5 W for 5 min. MiracleGro showed chemical oxygen demand (COD) solvation post-sonication of 66%, twice that of other growth media per cell ruptured; which was unexpected based on observed consistent biomass quality. Media differences do not appear to have an effect on ultrasound power transfer; thus, C. vulgaris grown in MiracleGro medium has a decreased strength in terms of resistance to rupture by ultrasound. These results suggest that while biomass productivity and composition are important for the efficiency of extraction, media effects on the susceptibility of cells to pretreatment should not be ignored in overall process design.

Copyright © 2018 by ASME
Topics: Ultrasound , Biomass
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Grahic Jump Location
Fig. 1

Calculated molar concentration of nitrogen, phosphorus, and potassium in synthetic freshwater media

Grahic Jump Location
Fig. 2

Sample spectrophotometric absorbance spectrum of C. vulgaris grown in BBM medium.

Grahic Jump Location
Fig. 3

Intrinsic growth rates (r) of C. vulgaris grown in specified media. Sample size (n) defines cultures that reached observable maximum growth rate in 31 days without crash. Error bars are one standard deviation.

Grahic Jump Location
Fig. 4

Power ultrasound effect on percentage viability of C. vulgaris grown in specified media. Change in percentage cell viability as PI fluorescence after 5 min 9.5 W power ultrasound treatment of 50 ml volume. Error bars are one standard deviation. Minimum viability of cultures prior to treatment was 98.2%.

Grahic Jump Location
Fig. 5

Solvation of C. vulgaris COD in specified media by power ultrasound. Soluble fraction of COD before and after 5 min 9.5 W power ultrasound treatment of 50 ml volume. Error bars are one standard deviation.



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