3D printing is a layer-by-layer deposition process, which results in highly anisotropic structures and contains interfaces. Complex shapes manufactured by 3D printing carry defects. Complete elimination of these defects and interfaces is not possible, and these defects degrade the mechanical properties. In the present study, mechanical properties of printed dog bone samples are quantified as a function of building parameters, in particular, filling patterns, raster angle, and orientation of build direction with respect to that of loading, in polylactic acid (PLA). The tensile strength of 3D-printed PLA is the same for hexagonal and linear pattern filling when the build direction is along thickness and width, and failure was initiated at the defects in the structure, while better overall toughness is offered by hexagonal pattern filling. Build direction along specimen gauge length gives very low tensile strength and toughness, and failure happens between the printing layers. To minimize the defects especially near the grip section, cuboid samples were first deposited and micro-machined by laser into dog bone shape to perform tension test. Tensile strength and elastic modulus of micro-machined samples are surprisingly lower, while failure strain is highest among line filling printed samples. Damage resistance was quantified in terms of work of fracture, and hexagonal filling provided better damage resistance than line filling patterns for conditions of 0 deg raster angle with respect to the crack, whereas line filling with 45 deg and 90 deg raster angle tolerated damage better than hexagonal filling.